Rapid-Lenti HIV-1 p24 ELISA Kit (Single Wash)
The Rapid-Lenti HIV-1 p24 ELISA Kit (Single Wash) is a high-sensitivity enzyme-linked immunosorbent assay (ELISA) designed for the quantification of HIV-1 p24 antigen in cell culture supernatants and lentiviral samples. This assay provides rapid and accurate viral titer determination, enabling efficient monitoring of HIV-1 in research applications.
Assay Principle:
Microtitration wells are pre-coated with a murine anti-HIV-1 p24 capture antibody, allowing for the binding of p24 antigens from test samples. A biotinylated anti-HIV-1 p24 antibody and streptavidin-HRP conjugate are added to form an immune complex. After a single wash step, the substrate tetramethylbenzidine (TMB) is introduced, producing a colorimetric signal proportional to the p24 concentration. The absorbance is measured spectrophotometrically, providing a quantitative readout of the sample's viral load.
Applications:
✔ Lentiviral Vector Quantification
✔ HIV-1 Viral Load Monitoring in research studies
✔ Gene Therapy & Vaccine Development
Kit Presentation
Materials Supplied :The reagents supplied in this pack are for research use only.
1-Coated microwell strips - 1 plate.
Plastic microtitration wells coated with antiHIV-1 p24 murine monoclonal antibody in foil pouch with desiccant - 96 wells.
2-Positive HIV-1 p24 Control (10ng/ml)-0.1 mL.
3-Lysis Buffer-6 mL.
4-Detector Antibody. Anti-HIV-1 p24 conjugated to biotin - 6mL.
5-Conjugate. Streptavidin conjugated to horseradish peroxidase enzyme containing 0.01% Bromonitrodioxane as preservative - 6 mL.
6-Wash Buffer (20X concentrated). Tris buffered saline pH 7.8-8.0, containing 0.05% Tween 20. Must be diluted before use - 30 mL.
7-Substrate solution. Tetramethylbenzidine - 12 mL.
8 Stop Solution. 1 N H2SO4 - 12 mL.
Storage and Stability
All reagents should be stored at 2-8 °C and should not be used beyond the expiration date on the label. Once opened, microtitration strips may be stored at 2-8 °C until the expiration date on the label, provided that desiccated conditions are maintained. Unused strips should be returned to their original foil pouch along with the sachet of desiccant. Secure open foil pouch using zip top before storage. The working strength Wash Buffer should not be stored for longer than 3 weeks at 2-8 °C . It is recommended that Wash Buffer be freshly diluted before each assay. If the working strength buffer becomes visibly cloudy or develops precipitate during the 3 weeks, do not use it.